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Since 1999 Kromaton has pioneered in developing Fast Centrifugal
Partition Chromatography (FCPC™) systems for the separation and purification of complex mixtures of natural, synthetic or biological/biotechnological origin.

KROMATON IS THE WORLD MARKET LEADER IN CENTRIFUGAL PARTITION CHROMATOGRAPHY (CPC) with more than 130 systems operating around the globe.

Kromaton’s technological expertise is powered by Rousselet Robatel
know-how in designing and manufacturing industrial centrifugal systems made of stainless steel.This guarantees effectiveness and robustness through state-of-the-art design.

WHAT IS FCPC ?

Fast Centrifugal Partition Chromatography (FCPC™) is a liquid/liquid purification technology for high-added-value compounds and molecules. It is a preparative chromatography technique based on the principle that both mobile and stationary phases are liquid. This, a biphasic solvent system is utilized and the stationary phase is maintained in the column by centrifugal force, while mobile phase percolates

through, thus presenting several advantages in comparison with conventional chromatography techniques :

• No solid packing material to dispose.
• High stationary phase ratio in the
column.
• No sample loss due to irreversible
adsorption.
• No denaturation of sensitive compounds.

• High throughput capacity.
• Moderate solvent consumption.
• Wide range of possible applications
in terms of polarity.
• Easy and predictable scale-up.
• Versatile operation (elution/extrusion,
normal/reverse-phase, pH-zone
refining, ion-exchange, dual mode,
gradient elution, etc).

PRINCIPLE OF OPERATION

Centrifugal Partition Chromatography operation relies on a simple principle. A biphasic solvent system is the core of this operation :

• One phase is selected to act as a
stationary phase, either the lighter or
the heavier.This is achieved by centrifugal force.

• The other phase is continuously
diffusing through, thus acting as the
mobile phase.This is achieved by a pumping system.

The solutes of interest are introduced into the chromatographic column and are migrating along its length according to their distribution coefficient between the mobile and stationary phase.The column (also called rotor) is specifically designed with twin-cells and ducts that guarantee optimal surface of contact between the two phases.

PRODUCTS

FCPC C – LABORATORY SCALE EQUIPMENT
FCPC C is a benchtop system, ideal for method development and preparative
applications involving small sample quantities, low solvent consumption and
faster separations.

Two interchangeable rotors can be mounted on this skid, 25 and 50ml.

FCPC A – VERSATILE & SCALABLE EQUIPMENT
FCPC A is a benchtop system, ideal for method development as well as preparative applications with upscalable results and manual or automated control powered by an integrated LC station.

Additionally it can take a Centrifugal Partition Extractor rotor (CPE)
of 300ml total volume.

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